Kinetically Controlled Synthesis of Cefaclor with Immobilized Penicillin Acylase in the Presence of Organic Cosolvents
نویسندگان
چکیده
Ye-Wang Zhang, Shu-Lai Liu, Dong-Zhi Wei, Rui-Jiang Liu, and Xi-Ming Xu School of Pharmaceutics, Jiangsu University, Zhenjiang, 212013, P. R. China State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, P. R. China College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310014, P. R. China United research center of Jiangsu University and Sopo Xingda Pharmaceutical Co. Ltd., Zhenjiang, 212013, Zhenjiang, P. R. China
منابع مشابه
Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase
Penicillin G acylase from E. coli TA1 was immobilized by Cross-Linked Enzyme Aggregates (CLEA), a new method for immobilization. This biocatalyst and commercial immobilized penicillin G acylase (PGA-450) were used to study the effect of pH, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). Compare...
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Penicillin G acylase from E. coli TA1 was immobilized by Cross-Linked Enzyme Aggregates (CLEA), a new method for immobilization. This biocatalyst and commercial immobilized penicillin G acylase (PGA-450) were used to study the effect of pH, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). Compare...
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The catalytic properties of penicillin G acylase (PGA) from Escherichia coli in kinetically controlled synthesis of β-lactam antibiotics are negatively affected upon immobilization on hydrophobic acrylic carriers. Two strategies have been here pursued to improve the synthetic performance of PGA immobilized on epoxy-activated acrylic carriers. First, an aldehyde-based spacer was inserted on the ...
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Covalently immobilised penicillin G acylase (PGA-450) accepts in toluene, at controlled water activity (aw), a broad range of amino compounds as nucleophiles in kinetically controlled acylation. Hydrolytic reactions were prevented and complete conversions were achieved in short times even when working with an equimolar concentration of the substrates. The recovery of the products was facile, le...
متن کاملNew active site oriented glyoxyl-agarose derivatives of Escherichia coli penicillin G acylase
BACKGROUND Immobilized Penicillin G Acylase (PGA) derivatives are biocatalysts that are industrially used for the hydrolysis of Penicillin G by fermentation and for the kinetically controlled synthesis of semi-synthetic beta-lactam antibiotics. One of the most used supports for immobilization is glyoxyl-activated agarose, which binds the protein by reacting through its superficial Lys residues....
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تاریخ انتشار 2008